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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis

Fig. 1

Determination of recombinant plasmids pEASY-FPV-H and pEASY-CPV-2a. The two plasmids were PCR from CPV JL14–1 strain and vaccine FPV with primers F1 and R1 and cloned to pEASY™-T5 zero vector, and then identified by PCR and sequencing. a Agarose gel (3%) showing the amplification of recombinant plasmids from FPV and CPV. Lane M: DL2000 (Takara Biotechnology Co., Ltd., Dalian, China); lane 1: Negative control; lane 2: pEASY-FPV-H; lane 3: pEASY-CPV-2a; lane 4: Positive DNA of CPV JL14–1 strain. b Alignment the sequences of the two plasmids. The SNP at the relevant position of pEASY-FPV-H and pEASY-CPV-2a are same to the original viruses, FPV, and CPV (A and C, respectively)

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BMC Veterinary Research

ISSN: 1746-6148

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