Use of a recombinase polymerase amplification commercial kit for rapid visual detection of Pasteurella multocida

Table 5 RPA primers and LF-probe designed in this study

Name	Sequence(5′-3′)	Genome location	Amplification size(bp)
Kmt1-F1	CGATTGCCGCGAAATTGAGTTTTATGCCAC	249–278	192
Kmt1-F2	TTGCCGCGAAATTGAGTTTTATGCCACTTG	252–281	189
Kmt1-F3	ATTGAGTTTTATGCCACTTGAAATGGGAA	262–290	179
Kmt1-R	Biotin-AATAACGTCCAATCAGTTGCGCCGTTGTCA	411–440
Kmt1-LF Probe	FITC-ATGGCATTATTTTATGGCTCGTTGTGAGTG [dSpacer]GCTTGTCGGTAGTCTT-C3 Spacers	291–337	150
Pm-F1	CGATTGCCGCGAAATTGAGT	249–268	241
Pm-F2	CAGAGTTTGGTGTGTTGA	378–395	113
Pm-R	CAGACTGACAAGGAAATATAAAC	468–490

Abbreviations: dSpacer is an exonuclease site, and C3 Spacers is a polymerase extension blocking site

ISSN: 1746-6148