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Table 2 Bacterial strains used to test RPA-LFD assay specificity

From: Use of a recombinase polymerase amplification commercial kit for rapid visual detection of Pasteurella multocida

Number

Species

Strains/origin

RPA-LFD

1

Pasteurella multocida (A)

BNCC126487a

Positive

2

Pasteurella multocida (A) JN14

Clinical separationb

Positive

3

Pasteurella multocida (A) TJ14

Clinical separationb

Positive

4

Pasteurella multocida (A) JN15

Clinical separationb

Positive

5

Pasteurella multocida (B)

BNCC126234a

Positive

6

Pasteurella multocida (B) TJ16

Clinical separationb

Positive

7

Pasteurella multocida (D)

CVCC392c

Positive

8

Pasteurella multocida (E)

CVCC393c

Positive

9

Pasteurella multocida (F)

CVCC394c

Positive

10

Pasteurella avium

BNCC128728a

Negative

11

Pasteurella gallinarum

BNCC131586a

Negative

12

Pasteurella trehalosi

TCCPTA-3668a

Negative

13

Pasteurella stomatis

ATCC43327a

Negative

14

Pasteurella langaa

ATCC43328a

Negative

15

Pasteurella dagmatis

ATCC51570a

Negative

16

Actinobacillus pleuropneumoniae

BNCC129808a

Negative

17

Mycoplasma bovis PG45

ATCC25523a

Negative

18

Mycoplasma mycoides subsp. mycoides

BNCC126186a

Negative

19

Mannheimia haemolytica

BNCC128674a

Negative

20

Trueperella pyogenes

Clinical separationb

Negative

21

Histophilus somni

Clinical separationb

Negative

22

Klebsiella pneumoniae

BNCC194477a

Negative

23

Proteus mirabilis

BNCC107943a

Negative

24

Streptococcus bovis

ATCC33317a

Negative

25

Staphylococcus aureus

ATCC6538Pa

Negative

26

Escherichia coli O157:H7

BNCC186579a

Negative

27

Pseudomonas aeruginosa

Clinical separationb

Negative

28

Salmonella typhimurium

BNCC108207a

Negative

29

Enterococcus faecalis

ATCC29212a

Negative

30

Bacillus cereus

BNCC103930 a

Negative

  1. aThese strains were provided from BNCC; bThese strains were preserved in our laboratory; cThese strains were provided from CVCC