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Table 1 Assay detection results from P. multocida plasmid DNA standards

From: Use of a recombinase polymerase amplification commercial kit for rapid visual detection of Pasteurella multocida

Number DNA standards (copies/μl) Real-time qPCR RPA-LFD
Ct 1 Ct 2 Ct 3 Test 1 Test 2 Test 3
1 60,000,000 13.82 13.95 13.92 + + +
2 6000,000 17.15 17.18 17.23 + + +
3 600,000 20.12 20.36 20.43 + + +
4 60,000 23.92 23.56 23.29 + + +
5 6000 26.81 27.07 26.31 + + +
6 600 28.19 28.1 28.22 + + +
7 60 31.47 31.86 31.09 + + +
8 6 40.00 40.00 39.07 +
9 NC 40.00 40.00 40.00
  1. Ct threshold cycle, NC negative control