Number | DNA standards (copies/μl) | Real-time qPCR | RPA-LFD | ||||
---|---|---|---|---|---|---|---|
Ct 1 | Ct 2 | Ct 3 | Test 1 | Test 2 | Test 3 | ||
1 | 60,000,000 | 13.82 | 13.95 | 13.92 | + | + | + |
2 | 6000,000 | 17.15 | 17.18 | 17.23 | + | + | + |
3 | 600,000 | 20.12 | 20.36 | 20.43 | + | + | + |
4 | 60,000 | 23.92 | 23.56 | 23.29 | + | + | + |
5 | 6000 | 26.81 | 27.07 | 26.31 | + | + | + |
6 | 600 | 28.19 | 28.1 | 28.22 | + | + | + |
7 | 60 | 31.47 | 31.86 | 31.09 | + | + | + |
8 | 6 | 40.00 | 40.00 | 39.07 | – | – | + |
9 | NC | 40.00 | 40.00 | 40.00 | – | – | – |