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Table 1 Assay detection results from P. multocida plasmid DNA standards

From: Use of a recombinase polymerase amplification commercial kit for rapid visual detection of Pasteurella multocida

Number

DNA standards (copies/μl)

Real-time qPCR

RPA-LFD

Ct 1

Ct 2

Ct 3

Test 1

Test 2

Test 3

1

60,000,000

13.82

13.95

13.92

+

+

+

2

6000,000

17.15

17.18

17.23

+

+

+

3

600,000

20.12

20.36

20.43

+

+

+

4

60,000

23.92

23.56

23.29

+

+

+

5

6000

26.81

27.07

26.31

+

+

+

6

600

28.19

28.1

28.22

+

+

+

7

60

31.47

31.86

31.09

+

+

+

8

6

40.00

40.00

39.07

+

9

NC

40.00

40.00

40.00

  1. Ct threshold cycle, NC negative control