TY - JOUR AU - Rubio, Camila Peres AU - Mainau, Eva AU - Cerón, José Joaquín AU - Contreras-Aguilar, Maria Dolores AU - Martínez-Subiela, Silvia AU - Navarro, Elena AU - Tecles, Fernando AU - Manteca, Xavier AU - Escribano, Damian PY - 2019 DA - 2019/05/14 TI - Biomarkers of oxidative stress in saliva in pigs: analytical validation and changes in lactation JO - BMC Veterinary Research SP - 144 VL - 15 IS - 1 AB - Biomarkers of oxidative stress in pigs have been measured in serum/plasma samples. However, blood collection in pigs can be highly stressful to the animals. Saliva is a biological fluid with several advantages in pigs over blood, since it can be easily collected without stress to the animals, being therefore an ideal sample in this species. The objective of this study was the validation of assays for the evaluation of oxidative stress status in saliva of pigs. For this purpose, three assays commonly used to evaluate the total antioxidant capacity (TAC): trolox equivalent antioxidant capacity (TEAC), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing ability of plasma (FRAP)), one individual antioxidant (uric acid) and two assays to evaluate oxidant concentrations (advanced oxidation protein products (AOPP) and hydrogen peroxide (H2O2)) were measured and validated in porcine saliva. In addition, the possible changes of these assays in sows’ saliva during lactation were be studied. SN - 1746-6148 UR - https://doi.org/10.1186/s12917-019-1875-z DO - 10.1186/s12917-019-1875-z ID - Rubio2019 ER -