Fig. 5

Effect of pVW-MSCs conditioned medium on endothelial cells angiogenesis. a: the presence of pVW-MSCs conditioned medium (CM) induced endothelial cells to form a capillary like network compared to unconditioned medium (UM). b: pAECs cultured in extracellular matrix coating were exposed to conditioned medium obtained from unstimulated pVW-MSCs (CM) or 0.1 and 10 μg/ml LPS treated pVW-MSCs (CM 0.1; CM 10) and from recovery (CM0.1R; CM10R). c: representative images of pAECs in vitro angiogenesis in the presence of UM, CM, CM10, CM10. Scale bar = 100 μm. d, e, f: the effect of conditioned medium on endothelial cells migratory capacity was demonstrated by scratch test. Confluent pAECs were scratched (T0) and exposed to UM and CM, CM0.1, CM10, CM0.1R, CM10R for 24 h (T1). All data shown represent the mean ± SD of three biological replicates, each experiment is repeated two times. Data (Fig. a, d, e) were analysed using Student’s t-test between different treatment (UM vs CM) or different time (T0 vs T1) for each treatment. Significant differences are indicated by *(p < 0.05) and **(p < 0.01). Data (Fig. b) was analysed using one-way ANOVA followed by the Tukey’s post hoc comparison test, different letters above the bars indicate significant differences (p < 0.05)