Fig. 3
Transcriptional profile of cytokine and chemokine in pVW-MSCs after LPS treatment a: cytokines and chemokines relative expression evaluated by RT2 Profiler™ PCR Array Pig Cytokines & Chemokines in pVW-MSCs cultured with LPS (10 μg/ml) for 4 h. b: qRT-PCR analysis of selected genes in pVW-MSCs cultured in control condition and with LPS 0.1 μg/ml or10 μg/ml for 4 h. Data represent the mean ± the range of relative expression of three biological replicates, each experiment is repeated two times. Data were analysed using one-way ANOVA followed by the Tukey’s post hoc comparison test. Different letters above the bars indicate significant differences (p < 0.05). The relative mRNA expression (fold of change) was calculated in relation to control cells using the 2-ΔΔct method. nd = not detectable