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Table 3 Antibodies and dilutions used in the analyses of NDRG1 in canine tissues and cells

From: Cell and context-dependent sorting of neuropathy-associated protein NDRG1 – insights from canine tissues and primary Schwann cell cultures

Name Catalog number Producer Dilutions
    IHC IF WB
Monoclonal anti-NDRG1 antibody produced in mouse WH0010397 Sigma-Aldrich, Merck, Darmstadt, Germany 1/2000 1/2000 1/2000
Polyclonal anti-NDRG1 antibody produced in goat PA5–18109 Invitrogen, Thermo Fisher Scientific 1/750 NA NA
Polyclonal anti-NDRG1 antibody produced in rabbit HPA006881 Sigma-Aldrich, Merck 1/100 1/100 1/1000
Monoclonal phospho-specific anti-NDRG1 (Thr346) produced in rabbit 5482 Cell Signaling Technology, Leiden, Netherlands NA 1/500 1/1000
Monoclonal phospho-specific anti-NDRG1 (Ser330) produced in rabbit ab124713 Abcam, Cambridge, United Kingdom NA 1/75 1/10000
Anti-glial fibrillary acidic protein Z0334 Dako, California, United States 1/500 1/500 NA
Anti-Iba1 019–19,741 Fujifilm Wako Chemicals, Neuss, Germany 1/250 NA NA
  1. IHC Immunohistochemistry, IF Immunofluorescence, WB Western blot, NA Not analyzed