Fig. 3

The levels of IL-1β and TNF-ɑ measured by ELISA. After treated with gradient concentrations of LPS in hoof dermal cells for 24 h and 48 h, levels of IL-1β (a) and TNF-ɑ (b) were detected (n = 8). (^**P-value< 0.01; ^*P-value< 0.05, compared with the control group). After treated with gradient concentrations of silymarin in LPS-induced hoof dermal inflammatory cells for 24 h and 48 h, levels of IL-1β (c) and TNF-ɑ (d) were detected (n = 8). (^**P-value< 0.01; ^*P-value< 0.05, compared with the LPS model group)