Characterisation of Pasteurella multocida isolates from pigs with pneumonia in Korea

Kim, Jongho; Kim, Jong Wan; Oh, Sang-Ik; So, ByungJae; Kim, Won-Il; Kim, Ha-Young

doi:10.1186/s12917-019-1861-5

BMC Veterinary Research

Table 1 Primers used for the detection of capsular types and virulence-associated genes in Pasteurella multocida isolates

From: Characterisation of Pasteurella multocida isolates from pigs with pneumonia in Korea

Gene function	Target gene	Description	Sequence (5′ – 3′)	size (bp)	Reference
Capsule serotypes	kmt1	Identification of all P. multocida isolates	ATCCGCTATTTACCCAGTGG GCTGTAAACGAACTCGCCAC	460	[19]
	hyaD-hyaC	Serogroup A cap gene	TGCCAAAATCGCAGTGAG TTGCCATCATTGTCAGTG	1044	[20]
	bcbD	Serogroup B cap gene	CATTTATCCAAGCTCCACC GCCCGAGAGTTTCAATCC	760	[20]
	dcbF	Serogroup D cap gene	TTACAAAAGAAAGACTAGGAGCCC CATCTACCCACTCAACCATATCAG	657	[20]
	ecbJ	Serogroup E cap gene	TCCGCAGAAAATTATTGACTC GCTTGCTGCTTGATTTTGTC	511	[20]
	fcbD	Serogroup F cap gene	AATCGGAGAACGCAGAAATCAG TTCCGCCGTCAATTACTCTG	851	[20]
Outer membrane and porin proteins	oma87	Outer membrane protein 87	ATGAAAAAACTTTTAATTGCGAGC TGACTTGCGCAGTTGCATAAC	948	[17]
	ompH	Outer membrane protein H	CGCGTATGAAGGTTTAGGT TTTAGATTGTGCGTAGTCAAC	438	[17]
	plpB	Outer membrane protein	TTTGGTGGTGCGTATGTCTTCT AGTCACTTTAGATTGTGCGTAG	282	[18]
	psl	Porin protein	TCTGGATCCATGAAAAAACTAACTAAAGTA AAGGATCCTTAGTATGCTAACACAGGACGACG	470	[17]
Adhesins	fimA	Fimbriae	CCATCGGATCTAAACGACCTA AGTATTAGTTCCTGCGGGTG	866	[18]
	pfhA	Filamentous haemagglutinin	AGCTGATCAAGTGGTGAAC TGGTACATTGGTGAATGCTG	275	[21]
	ptfA	Fimbriae	TGTGGAATTCAGCATTTTAGTGTGTC TCATGAATTCTTATGCGCAAAATCCTGCTGG	488	[17]
	hsf-1	Autotransporter adhesion	TTGAGTCGGCTGTAGAGTTCG ACTCTTTAGCAGTGGGGACAACCTC	654	[18]
	hsf-2	Autotransporter adhesion	ACCGCAACCATGCTCTTAC TGACTGACATCGGCGGTAC	433	[18]
Superoxide dismutases	sodA	Superoxide dismutases	TACCAGAATTAGGCTACGC GAAACGGGTTGCTGCCGCT	361	[17]
Superoxide dismutases	sodC	Superoxide dismutases	AGTTAGTAGCGGGGTTGGCA TGGTGCTGGGTGATCATCATG	235	[17]
Iron acquisition related factor	exbB	Iron regulated and acquisition factors	TTGGCTTGTGATTGAACGC TGCAGGAATGGCGACTAAA	283	[18]
	exbBD-tonB	Iron acquisition related factors	GGTGGTGATATTGATGCGGC GCATCATGCGTGCACGGTT	1144	[17]
	fur	Iron regulated and acquisition factors	GTTTACCGTGTATTAGACCA CATTACTACATTTGCCATAC	244	[18]
	tbpA	Iron acquisition related factor	TGGTTGGAAACGGTAAAGC TAACGTGTACGGAAAAGCC	728	[21]
	hgbA	Haemoglobin binding protein	TGGCGGATAGTCATCAAG CCAAAGAACCACTACCCA	419	[17]
	hgbB	Haemoglobin binding protein	TCATTGAGTACGGCTTGAC CTTACGTCAGTAACACTCG	499	[21]
Neuraminidases	nanB	Neuraminidases	GTCCTATAAAGTGACGCCGA ACAGCAAAGGAAGACTGTCC	554	[17]
Neuraminidases	nanH	Neuraminidases	GAATATTTGGGCGGCAACA TTCTCGCCCTGTCATCACT	360	[17]
Hyaluronidase	pmHAS	Hyaluronidase	TCAATGTTTGCGATAGTCCGTTAG TGGCGAATGATCGGTGATAGA	430	[18]
Toxin	toxA	Dermonecrotic toxin	TCTTAGATGAGCGACAAGG GAATGCCACACCTCTATAG	846	[21]

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ISSN: 1746-6148

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