Time | Groups and number of embryos/chickens | Action | ||||
---|---|---|---|---|---|---|
Pre1 | Pre2 | Syn1 | Syn2 | C | ||
E12 | 800 | live embryos | ||||
E12 | 160 | 160 | 160 | 160 | 160 | embryos given to experimental groups and treated in ovo |
D1 | 145 | 137 | 114 | 145 | 130 | hatched chickens |
D7 | − 7 | −7 | −7 | − 7 | − 7 | randomly selected chickens killed and used to tests |
D21 | −7 | − 7 | − 7 | − 7 | − 7 | randomly selected chickens killed and used to tests |
D35 | −7 | − 7 | − 7 | − 7 | − 7 | randomly selected chickens killed and used to tests |