Table 1 Primer sequences for common porcine gastrointestinal pathogens
Pathogens | primers(5′-3′) | product length (base pair) | GenBank accession no. |
|---|---|---|---|
L. intracellularis | F:AAATCCAAAAGTCGAGTATCTAACTGCGG | 292 | AM180252 |
R:TAAAAACCCAGAGCAAAATCGTGATACCAGGCG | Â | ||
E. coli | F:AACGGTTGCTCTTCATTTAG | 678 | CP034794 |
R:GACCATCCAATAAGTGTG | Â | ||
S. Cholerasuis | F:GCTCTTTCGTCTGGCATTA | 351 | CP034819 |
R:AACTTCATCGCACCGTCA | Â | ||
E. faecalis | F:AAAGTAGAATTAGATCCACAC | 320 | CP031027 |
R:TCTATCACATTCGGTTGCG | Â | ||
B. hyodysenteriae | F:ACTAAAGATCCTGATGTATTTG | 352 | CP019600 |
R:CTAATAAACGTCTGCTGC | Â | ||
PCV2 | F:GGTGCCCGCTGCCACATCGAGAAAG | 589 | MH718995 |
R:AGCTTCTACAGCTGGGACAGCAG | Â | ||
PRV | F:GAGTACGTCACGGTCATCAAGGAG | 553 | NC006151 |
R:CACTTCCGGTTTCTCCGGATC | Â | ||
PoRV | F:GGCTTTAAAAGAGAGAATTTCCG | 1062 | JF835112 |
R:GGTCACATCATACAATTCTAA | Â | ||
PEDV | F:CATGGGCTAGCTTTCAGGTC | 182 | MK288006 |
R:CGGCCCATCACAGAAGTAGT | Â | ||
TGEV | F:TCTTTCTTCTACCCTATTATGACTG | 1117 | KT696544 |
R:CTYTGGAGTATGGTGGTGTTC | Â | ||
CSFV | F:GGTGGTCTAAGTCCTGAGTACAGG | 177 | MK121886 |
R:GCCTCTGCAGCACCCTATCAGGTCG | Â |