Skip to main content
Fig. 2 | BMC Veterinary Research

Fig. 2

From: Rapid and visual detection of Lawsonia intracellularis with an improved recombinase polymerase amplification assay combined with a lateral flow dipstick

Fig. 2

Specificity of the RPA-LFD assay. a The analytical specificity test demonstrated that DNAs from E. coli, Salmonella Cholerasuis (S. Cholerasuis), Enterococcus faecalis (E. faecalis), Brachyspira hyodysenteriae (B. hyodysenteriae), porcine circovirus serotype 2 (PCV2), pseudorabies virus (PRV) and porcine rotavirus (PoRV), and RNAs from porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), classical swine fever virus (CSFV), and sample containing the above pathogens expect for L. intracellularis showed no cross-reactions in the developed L. intracellularis RPA-LFD (Lane 2–12), only sample containing both L. intracellularis and the above pathogens produced positive Test line (Lane 1); b Specific amplification L. intracellularis, E. coli, S. Cholerasuis, E. faecalis, B. hyodysenteriae, PCV2, PRV, PoRV, PEDV, TGEV, and CSFV by PCR using nucleic acids extracted from sample containing the above pathogens (Lane 1–12)

Back to article page

BMC Veterinary Research

ISSN: 1746-6148

Contact us