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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Rapid and visual detection of Lawsonia intracellularis with an improved recombinase polymerase amplification assay combined with a lateral flow dipstick

Fig. 1

Specific amplification of dnaA gene and optimization of condition for L. intracellularis RPA-LFD. The dnaA gene fragment of L. intracellularis was specifically amplified by recombinase polymerase amplification (RPA) using designed primers (a); Concentration of primers (b), reaction temperature (c), and reaction time (d) were optimized for RPA, and the amplicons were detected using lateral flow dipstick (LFD). M: DL2000 DNA Marker; 1: L. intracellularis genomic DNA; 2: E. coli genomic DNA; 3: Blank control. L. intracellularis DNA positive samples (+) and negative samples (−) were used as templates in RPA, respectively

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