Table 4 The primers used for PCR amplification of Y chromosome-specific probe
From: A dual colour FISH method for routine validation of sexed Bos taurus semen
Size of PCR product | Type of primer | Sequence of primer 5′ - > 3’ | |
|---|---|---|---|
btDYZ1 | 243 bp | Forward | TGTAGATGTGTGTGCCATCC |
Reverse | ACCGGTTCCACAGTCTCTAG |