Skip to main content

Table 3 The primers used for PCR amplifications of X chromosome-specific probes

From: A dual colour FISH method for routine validation of sexed Bos taurus semen

 

Size of PCR product

Type of primer

Sequence of primer 5′ - > 3’

X1

234 bp

Forward

CTGCTGTGGCTTCCTGGTTA

Reverse

GTATCATGGCCTCCCTCAGC

X2

532 bp

Forward

GTCAACGGAGGTACAGAGGC

Reverse

AGCAGACCTCTGGAGACACA

X3

555 bp

Forward

TGGCCAACCAGGAAAAGACT

Reverse

TGGGACTGCTAATTGTGGGT

X4

203 bp

Forward

CATGAGAAGAAACACCATGCCC

Reverse

CCACACCCTTCAATCTTGGTCAG

X5

275 bp

Forward

GTCAGTCCTGCAACAGGGAA

Reverse

TCTGGCACTTTAAATACTGAGAGAC