A detection of benzimidazole resistance-associated SNPs in the isotype-1 β-tubulin gene in Haemonchus contortus from wild blue sheep (Pseudois nayaur) sympatric with sheep in Helan Mountains, China

Table 1 Number and frequency (%) with 95% CI^a of individual worm genotypes with “susceptible” and “resistant” single nucleotide polymorphism (SNP) and the allele frequency (%) with 95% CI at codon E198A (GAA/GCA) associated with benzimidazole resistance in isotype-1 β-tubulin gene of H. contortus worms from sympatric blue sheep and sheep in Helan Mountains, China

Hosts	Number of genotype^b and frequency (%) with 95% CI Frequency^c (%) with 95% CI for codon E198A
Hosts	Hs-198	Hr-198	Het-198	Susceptible	Resistant
Sheep	17 (85.00, 63.96~94.76)	0 (0.00, 0.00~16.11)	3 (15.00, 5.24~36.04)	(92.50, 80.14~97.42)	(7.50, 2.58~19.86)
Blue sheep	23 (85.20, 67.52~94.08)	2 (7.40, 2.06~23.37)	2 (7.40, 2.06~23.37)	(88.89, 77.81~94.81)	(11.11, 5.19~22.19)

^a CI Confidence interval
^b Genotype: Hs, homozygous susceptible; Hr, homozygous resistant; Het, heterozygote
^c The allele frequency for codon E198A (%) was calculated using as below: Susceptible of sheep = (Hs-198× 2 + Het-198)/(20 × 2). Resistant of sheep = (Hr-198× 2 + Het-198)/(20 × 2). Susceptible of blue sheep = (Hs-198× 2 + Het-198)/(27 × 2). Resistant of blue sheep = (Hr-198× 2 + Het-198)/(27 × 2)

ISSN: 1746-6148