Identification and genomic analysis of two novel duck-origin GPV-related parvovirus in China

Table 1 Primers used to amplify the complete genome

Primers	Sequences	Position	Size of PCR products (bp)	Annealing temperature
P1-F	CTCATTGGAGGGTTCGTTCG	1–20	191	58 °C
P1-R	GCATGCGCGTGGTCAACCTAACA	169–191	191	58 °C
P2-F	CAAACGGGGAGGGCAAAATAAGA	186–208	1345	60 °C
P2-R	GTGGTCGCAGGTCCGTAGAGC	1511–1531	1345	60 °C
P3-F	GGGTGAAGAGAGAGTTCAACAA	1474–1497	1137	52 °C
P3-R	CGTTACCAGGCCCAAGATAC	2591–2610	1137	52 °C
P4-F	GGCATTTGAAAGCTGGAGCC	2473–2492	922	60 °C
P4-R	GTCTTCGTCTGATCCTGCGT	3375–3395	922	60 °C
P5-F	TTGCGATTCCCAATGGATG	3089–3107	1171	58 °C
P5-R	CCCAAATAGGTCCCTGTAGATA	4239–4260	1171	58 °C
P6-F	CTACAACCCGGACCTGTGTC	3948–3967	921	60 °C
P6-R	GCATGCGCGTGGTCAACCTAACA	4846–4868	921	60 °C