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Table 1 The effect of TC (41 °C for 6 h; Post- Hatch Day 14) during 4 time terminals (at 0, 1, 3 and 5 h of TC) on relative mRNA expression of Hsp108, Hsp90, HSF-1 and HSF-2 in the Muscle of broiler chicks subjected to different TM protocols at ED 12–18 (n = 5)

From: Basal and dynamics mRNA expression of muscular HSP108, HSP90, HSF-1 and HSF-2 in thermally manipulated broilers during embryogenesis

Parameters

Groups

Treatments

 

Control

TM1

TM2

TM3

TM4

Hsp108

TN

1.00 ± 0.02aw

2.96 ± 0.03bw

1.63 ± 0.01cw

1.53 ± 0.01cw

2.28 ± 0.03dw

 

TC 1

4.87 ± 0.02ax

5.09 ± 0.08bx

6.37 ± 0.07cx

3.48 ± 0.08dx

11.9 ± 0.27ex

 

TC 3

6.86 ± 0.12ay

8.98 ± 0.06by

6.80 ± 0.06 ay

8.33 ± 0.04by

6.22 ± 0.01cy

 

TC 5

1.40 ± 0.03az

1.47 ± 0.05az

1.58 ± 0.06bw

1.24 ± 0.07cz

1.82 ± 0.04dz

Hsp90

TN

1.00 ± 0.01aw

2.90 ± 0.01bw

1.57 ± 0.02cw

1.35 ± 0.02dw

2.32 ± 0.03ew

 

TC 1

5.10 ± 0.03ax

4.74 ± 0.06bx

6.15 ± 0.06cx

3.22 ± 0.06dx

12.1 ± 0.29ex

 

TC 3

6.96 ± 0.32ay

8.54 ± 0.05by

6.54 ± 0.16ax

7.55 ± 0.04cy

6.06 ± 0.07dy

 

TC 5

1.50 ± 0.06az

1.42 ± 0.04az

1.80 ± 0.01bw

1.35 ± 0.06aw

1.85 ± 0.05bz

HSF-1

TN

1.00 ± 0.02aw

1.07 ± 0.02aw

0.99 ± 0.01aw

1.36 ± 0.05bw

0.98 ± 0.02aw

 

TC 1

0.56 ± 0.08ax

0.40 ± 0.07ax

1.61 ± 0.05bx

0.85 ± 0.04cx

2.84 ± 0.06dx

 

TC 3

0.98 ± 0.02aw

0.93 ± 0.06ay

1.21 ± 0.05by

0.99 ± 0.03cx

0.95 ± 0.04cw

 

TC 5

0.79 ± 0.01az

1.47 ± 0.05bz

1.01 ± 0.04cw

0.97 ± 0.02cx

1.28 ± 0.04dy

HSF-2

TN

1.00 ± 0.04aw

3.13 ± 0.07bw

1.48 ± 0.01cw

1.39 ± 0.03cw

2.09 ± 0.04dw

 

TC 1

1.65 ± 0.04ax

1.21 ± 0.04bx

2.12 ± 0.06cx

1.26 ± 0.08bw

3.02 ± 0.05dx

 

TC 3

1.55 ± 0.06ax

2.56 ± 0.08by

1.56 ± 0.07aw

1.71 ± 0.02cx

0.75 ± 0.02dy

 

TC 5

0.29 ± 0.01ay

1.97 ± 0.06bz

2.41 ± 0.09cy

2.48 ± 0.08cy

1.67 ± 0.05dz

  1. TC Thermal challenged, TM thermal manipulation, TN Naїve (thermal-neutral). Control = 37.8°C; TM1 = Thermal manipulation at 38.5°C for 18 h; TM2 = Thermal manipulation at 39 °C for 18 h; TM3 = Thermal manipulation at 39.5°C for 18 h; TM4 = Thermal manipulation at 40 °C for 18 h
  2. a–ewithin rows, means ± SD with different superscripts differ significantly (P < 0.05)
  3. w-zBetween naïve and TC chicks within a parameter, means ± SD with different superscripts differ significantly (P < 0.05)