Development and application of multiplex PCR method for simultaneous detection of seven viruses in ducks

BMC Veterinary Research

Table 1 Primers used in this study

Viruses	Primer sequences (5′-3′)	Targeted genes	Position	Accession no.	Product size (bp)	Working concentration (μM)
FAdV	F: CAACAGCCTCTCGTACCCAG	Hexon	839–858	AJ459805	102	0.12
FAdV	R: CCGATGTAGTTGGGCCTGAG	Hexon	921–940	AJ459805	102	0.12
DHAV	F: CTTTCCACTCCCTGCTCCC	VP1	510–528	KJ606043	140	0.12
DHAV	R: TTGGCTTCCACATCCTCTTCA	VP1	629–649	KJ606043	140	0.12
DEV	F: ATCGCATGTAGACGTTGGTT	UL2	726–745	EU885419	172	0.16
DEV	R: AGACAGCGGTGATGGATGG	UL2	879–897	EU885419	172	0.16
DTMUV	F: AATCGGTAGTGGCTTTGG	Envelope	432–449	AB110495	288	0.28
DTMUV	R: AGTCTGCCGACATGGATAT	Envelope	701–719	AB110495	288	0.28
NDV	F: CACCGGCAACCCTATTCTGT	Fusion	853–872	M24701	330	0.28
NDV	R: AGTGCGCCTTCAGTCTTTGA	Fusion	1163–1182	M24701	330	0.28
AIV	F: GGCGACTACTACCAACCCA	Matrix	521–539	DQ064401	435	0.24
AIV	R: CTGCTGTTCCTGCCGATAT	Matrix	937–955	DQ064401	435	0.24
NDPV	F: TATGTCCTGGGCTCGGCTAC	VP3	448–467	EF014903	516	0.24
NDPV	R: AGCTGACACAGGTCCAGGTT	VP3	944–963	EF014903	516	0.24

ISSN: 1746-6148