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Table 1 Overview of PCR methods in literature for detection of M. bovis

From: A European interlaboratory trial to evaluate the performance of different PCR methods for Mycoplasma bovis diagnosis

Method Characteristics Targeted gene Published by
End-point PCR For detection of M. bovis in milk samples and nasal swabs oppD/F [36]
For detection of M. bovis and M. agalactiae in diagnostic samples uvrC [26]
Design of a species-specific PCR assay for the identification and differentiation of M. agalactiae and M. bovis polC [25]
Development of a M. bovis species-specific PCR assay. Vsp [37]
PCR-DGGE PCR followed by denaturing gradient gel electrophoresis (DGGE) fingerprinting. For the simultaneous detection of mixed mycoplasma populations including M. bovis. 16S rRNA [27, 35, 38]
DNA microarray For parallel detection of 37 Mycoplasma species, including M. bovis. 23S rRNA; tuf [39]
Real-time PCR For quantifying the load of M. bovis in milk, nasal and conjunctival samples from cattle. oppD [23]
For pathogens associated with BRD, including M. bovis. oppD [40]
For detection of M. bovis in diagnostic samples. uvrC [24, 41, 42]
For detection of M. bovis in bovine milk samples fusA [43]
For detection of M. bovis in bovine milk and lung samples. 16S rRNA [44]
For detection of M. bovis, M. dispar and M. bovirhinis in bronchoalveolar lavage fluid from calves. 16S rRNA [22]