Table 1 Overview of PCR methods in literature for detection of M. bovis

End-point PCR

For detection of M. bovis in milk samples and nasal swabs

oppD/F

[36]

For detection of M. bovis and M. agalactiae in diagnostic samples

uvrC

[26]

Design of a species-specific PCR assay for the identification and differentiation of M. agalactiae and M. bovis

polC

[25]

Development of a M. bovis species-specific PCR assay.

Vsp

[37]

PCR-DGGE

PCR followed by denaturing gradient gel electrophoresis (DGGE) fingerprinting. For the simultaneous detection of mixed mycoplasma populations including M. bovis.

16S rRNA

[27, 35, 38]

DNA microarray

For parallel detection of 37 Mycoplasma species, including M. bovis.

23S rRNA; tuf

[39]

Real-time PCR

For quantifying the load of M. bovis in milk, nasal and conjunctival samples from cattle.

oppD

[23]

For pathogens associated with BRD, including M. bovis.

oppD

[40]

For detection of M. bovis in diagnostic samples.

uvrC

[24, 41, 42]

For detection of M. bovis in bovine milk samples

fusA

[43]

For detection of M. bovis in bovine milk and lung samples.

16S rRNA

[44]

For detection of M. bovis, M. dispar and M. bovirhinis in bronchoalveolar lavage fluid from calves.

16S rRNA

[22]