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Fig. 2 | BMC Veterinary Research

Fig. 2

From: A European interlaboratory trial to evaluate the performance of different PCR methods for Mycoplasma bovis diagnosis

Fig. 2

Determination of the analytical sensitivity of various M. bovis PCR assays in use by six laboratories (Lab 1 to Lab 6) on spiked bronchoalveolar lavage fluid (BALF) samples. Ten-fold serial dilutions (n = 7) of a mixture of 1 × 108M. bovis CFU/ml were prepared in BALF resulting in a range of 1 × 107 to 1 × 101M. bovis CFU/ml and tested by the various PCR methods. Viability of M. bovis in the BALF samples was verified with culture. Raw data to this figure are provided in Additional file 1

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