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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Effects of bovine tumor necrosis factor alpha decoy receptors on cell death and inflammatory cytokine kinetics: potential for bovine inflammation therapy

Fig. 2

Avidities of TNFR1 and R2-Ig to bovine TNF-α The avidities of TNFR-Ig to bovine TNF-α were analyzed using ELISA using biotinylated anti-bovine TNF-α antibody. Briefly, TNFR-Igs or Cont-Ig was fixed on ELISA plate and recombinant bovine TNF-α (0.098–25 ng/ml) was added. After the incubation (room temperature) and washing, biotinylated anti-bovine TNF-α antibody was added. TNFR2-Ig showed significantly higher avidity to bovine TNF-α than that showed by TNFR1-Ig (n = 3), * p < 0.05

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