Table 1 Optimal dilutions of coating antigen and serum sample antibodies for ELISA

50× (+)

1.043 ± 0.021

1.076 ± 0.028

1.306 ± 0.061

1.252 ± 0.068

1.288 ± 0.060

50× (−)

0.189 ± 0.006

0.213 ± 0.002

0.293 ± 0.004

0.283 ± 0.012

0.352 ± 0.087

P/N

5.519

5.051

4.457

4.424

3.659

100× (+)

0.812 ± 0.043

1.144 ± 0.032

1.223 ± 0.159

1.213 ± 0.086

1.086 ± 0.090

100× (−)

0.186 ± 0.005

0.197 ± 0.023

0.269 ± 0.024

0.286 ± 0.050

0.323 ± 0.098

P/N

4.366

5.807

4.584

4.241

3.362

150× (+)

0.800 ± 0.069

1.044 ± 0.076

0.922 ± 0.006

0.876 ± 0.048

0.986 ± 0.030

150× (−)

0.173 ± 0.016

0.185 ± 0.033

0.235 ± 0.001

0.256 ± 0.023

0.313 ± 0.008

P/N

4.624

5.643

3.923

3.422

3.150

200× (+)

0.742 ± 0.064

1.019 ± 0.146

0.776 ± 0.130

1.143 ± 0.073

1.125 ± 0.009

200× (−)

0.163 ± 0.010

0.241 ± 0.051

0.225 ± 0.001

0.273 ± 0.002

0.276 ± 0.009

P/N

4.552

4.228

3.449

4.187

4.076