Fig. 5From: Effects of divergent selection upon adrenocortical activity on immune traits in pigSpecific variations in lymphocyte subpopulations of HPAlo and HPAhi piglets. Lymphocyte subpopulations were characterized in EDTA-treated blood of 6-week old HPAlo (empty squares) and HPAhi (black squares) piglets (n = 16 per group) by multicolor staining using flow cytometry. Seven populations were identified: B lymphocytes (CD3− CD21+), naive CD4+ Th cells (CD3+ CD4+), antigen-experienced Th cells (CD3+ CD4+ CD8α+), CD8α− γδ T cells (CD3+ CD4− CD8α−), CD8α+ γδ T cells (CD3+ CD4− CD8αmed), CD8αhi cytotoxic T lymphocytes (CD3+ CD4− CD8αhi) and NK cells (CD3− CD4− CD8αmed); gating strategy is shown in Additional file 3: Figure S2. Each point represents a single piglet. Stars denote statistical significance (*p < 0.05; ** p < 0.01; ***p < 0.001)Back to article page