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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Effects of divergent selection upon adrenocortical activity on immune traits in pig

Fig. 5

Specific variations in lymphocyte subpopulations of HPAlo and HPAhi piglets. Lymphocyte subpopulations were characterized in EDTA-treated blood of 6-week old HPAlo (empty squares) and HPAhi (black squares) piglets (n = 16 per group) by multicolor staining using flow cytometry. Seven populations were identified: B lymphocytes (CD3 CD21+), naive CD4+ Th cells (CD3+ CD4+), antigen-experienced Th cells (CD3+ CD4+ CD8α+), CD8α γδ T cells (CD3+ CD4 CD8α), CD8α+ γδ T cells (CD3+ CD4 CD8αmed), CD8αhi cytotoxic T lymphocytes (CD3+ CD4 CD8αhi) and NK cells (CD3 CD4 CD8αmed); gating strategy is shown in Additional file 3: Figure S2. Each point represents a single piglet. Stars denote statistical significance (*p < 0.05; ** p < 0.01; ***p < 0.001)

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