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Table 2 The scoring system used to evaluate the inflammatory and repair phases of myofibre regeneration

From: Dexamethasone-induced impairment of post-injury skeletal muscle regeneration

Category Evaluation criterion Points assigned per criterion
Extravasation Absent 0
Focal 1
Extensive 2
Necrosis (myofibre hypercontraction, hyalinization and fragmentation of cytoplasm, loss of striations) Absent 0
Single fibres 1
Focal 2
Extensive 3
Inflammation (all inflammatory cells, i.e., neutrophils macrophages, lymphocytes, and eosinophils) Absent 0
Minimal: ≤ 5 inflammatory cells per HPF (40x) 1
Mild: 6–10 inflammatory cells per HPF (40x) 2
Moderate: 11–20 inflammatory cells per HPF (40x) 3
Marked: > 20 inflammatory cells per HPF (40x) 4
Myogenic precursor cells (MPCs) Absent 0
Not numerous: ≤ 3 MPCs per HPF (40x) 1
Moderately numerous: 4–6 MPCs per HPF (40x) 2
Numerous: 7–12 MPCs per HPF (40x) 3
Marked numerous: > 12 MPCs per HPF (40x) 4
Myotubes Absent 0
Not numerous: < 3 myotubes per HPF (40x) 1
Moderately numerous: 3–5 myotubes per HPF (40x) 2
Numerous: > 5 myotubes per HPF (40x) 3
Young myofibres Absent 0
Not numerous: < 3 young myofibres per HPF (40x) 1
Moderately numerous: 3–5 young myofibres per HPF (40x) 2
Numerous: > 5 young myofibres per HPF (40x) 3