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Table 1 Summary of the immunohistochemical methodology

From: Dexamethasone-induced impairment of post-injury skeletal muscle regeneration

Primary antibody Clone Dilution Antigen retrieval Visualization system
Anti-MyoD1a 5.8A 1:50 2 × 3 minb, Tris-EDTA buffer pH 9.03 min EnVision + System-HRP, Mouse (DAB)a
Anti-Desmina D33 1:50 2 × 3 minb, Tris-EDTA buffer pH 9.03 min EnVision + System-HRP, Mouse (DAB)a
  1. aDAKO, Glostrup, Denmark; bAntigen retrieval was conducted in a microwave oven at 650 W