Fig. 6
(a and b) Relative Oc and Mgp gene expression levels in Senegalese sole during larval development, in response to the different malathion treatments. Note in exposed speimens a strong down regulation of Oc expression from 15 dph onwards (a) and a moderate down regulation of Mgp expression levels from 13 dph onwards (b) when comparing with controls; c to h AA-AR staining in Senegalese sole at 30 dph showing for controls almost all calcified (red colour) structures c, b and e whereas for 6.25 μg/L malathion exposure, still remained blue in colour, indicating a cartilaginous composition (f, g and h)