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Table 4 PCR primer pairs used to amplify the feline CYP2R1 and CYP27B1 genes

From: A genetic variant of CYP2R1 identified in a cat with type 1B vitamin D-dependent rickets: a case report

PCR Product

Forward Primer (5′-3′)

Reverse Primer (5′-3′)

CYP2R1

 exon 1 exon 1

GCACTGTAGACTCTGAGCGG

TCACTTGCACAGAGGGTTGG

 exon 2 exon 2

AGAGCTGGACTTAGCACATG

TGATTCAAGTGTGCATGCAC

 exon 3 former

TGAGTAGCAGAGAACAGGGC

GCATCAACGAAATGCTGAGGT

 exon 3 latter

AGTGCCTCCGTCTTCCTGTA

AGAAACTGGCGTTCAGGTCC

 exon 4 exon 4

CTGGTTGTCCCCATACCCTG

CGTCCTGAAGTTATGCCCCA

 exon 5 exon 5

CACTGAGCTGAAGTGGGACA

CATCCACGGCCCTTCTTACT

CYP27B1

 exon 1 exon 1

AGAGGGGGCGTCACCACTATCC

CCGGCAGCCCCAATTTCTCTAT

 exon 2 exon 2

CTTGCACAGCCCAGCGAACACC

GGAAGCCCCATTTACCAGAAGG

 exon 3 exon 3

CCGGGGCAGGAAATGAGTAAC

CAGGCAGCCCAGGCGTGAACC

 exon 4 exon 4

GGGATGTGGCGGGAGAGTTTTA

TGGGGAGAAGGTACAGGGATGC

 exon 5 exon 5

CCTGGAGGCCGAAGTGC

CCAACAGGGAGGAGAACC

 exon 6 exon 6

GAGCCCAACTTCCAGAGC

CCAGGCCTTCCCAGCATTTT

 exon 7 exon 7

AAGGCGGTGGTCAAGGAAGTG

CAGGCCAAAGGGAGGGTGAA

 exon 8

TCCAAAAGTAGCCCCAGAA

TATTTTGCTATGCTTGTCAGAA

 exon 9

TCGACCAACCTAACACTAATAA

AGAAAGGATGAGCAGACACA