Table 1 Primers used in this study
From: The unfolded protein response induced by Tembusu virus infection
Primer name | Sequence | Primer name | Sequence |
|---|---|---|---|
GRP78-F | 5’-TCATCGGACGCACTTGGAA-3’ | Calreticulin-F | 5’-GGAGCCTGCCGTCTACTTC-3’ |
GRP78-R | 5’-TAGTGAGAACCATGGCAGAA-3’ | Calreticulin-R | 5’-GGTCTGGCCCTTGTTACTGA-3’ |
GRP94-F | 5’-CCGAGTTTGATGGGAAGAGGTT-3’ | ERp57-F | 5’-CTAGGACTGCCGATGGGATTGT-3’ |
GRP94-R | 5’-GGCCACAAGAGCACAAGGAGAT-3’ | ERp57-R | 5’-AGTTGCTGGCTGCTTTTAGGAA-3’ |
ATF4-F | 5’-AGCAAAACAAGACAGCAGCCACTA-3’ | PDI-F | 5’-CCCCGGAGGAGGAGGACAAC-3’ |
ATF4-R | 5’-TTGCCTTACGGACCTCCTCTATCA-3’ | PDI-R | 5’-CACACCACGGGGCATAGAAC-3’ |
GADD34-F | 5’-AGCAGCTGACCGAGGCAAGAG-3’ | CAS3-F | 5’-GCCCAAACTCTTCATCATTC-3′ |
GADD34-R | 5’-TTAGGGGCGGTCCAAGGTGA-3’ | CAS3-R | 5’-TCGGCTTCCACTGGTATCTT-3’ |
CHOP-F | 5’-AAGAGGAAGATCAAGGAAGAACTA-3’ | CAS8-F | 5’-CCTCATCAATCGGCTGGAC-3’ |
CHOP-R | 5’-CCATGCGGTCAATCAGAG-3’ | CAS8-R | 5’-ATGACCCTGTAGGCAGAAACC-3’ [58] |
XBP1-F | 5’-GAGAAGGCGCTGCGGAGGAAACTG-3′ | CAS9-F | 5’-CTCGAGGCAGGGACTTAGACA-3′ |
XBP1-R | 5’-GAGAAAGGGAGGCTGGTAAGGAAC-3′ | CAS9-R | 5’-AAACTTGACACGGCATCCA-3′ [59] |
P58-F | 5’-AGATGGCGACCCTGATAACTA-3′ | BHK-GAPDHF | 5’-ACTTGGCACATGTCTGTATGC-3′ |
P58-R | 5’-GACTGGGCTTCCTTCTCTTC-3′ | BHK-GAPDHR | 5’-CACCAGCATCACCCCATTT-3’ |
Calnexin-F | 5’-TGCCGAGCCAGGTGTAGTG-3’ | DF1-GRP94-F | 5’-CTGAGAAGTTTGCCTTTCAAGCAG-3’ |
Calnexin-R | 5’-CCTCTTCATCCCCCTTGTTCTT-3’ | DF1-GRP94-R | 5’-GCTCCTCATTACCAGCAAGAGCAT-3’ [60] |
DF1-uXBP1-F | 5’-CAGCACTCAGACTACGTGTTCCTCTG-3′ | DF1-sXBP1-F | 5’-GCTGAGTCCGCAGCAGG-3′ |
DF1-uXBP1-R | 5’-CTGCCATCAGAATCCATGTG-3’ [60] | DF1-sXBP1-R | 5’-CTGCCATCAGAATCCATGTG-3’ [60] |
DF1-ATF4-F | 5’-CAATTGGCTCGCTGTGGACAGTTT-3′ | DF1-GRP78-F | 5’-TGTAGCCTATGGTGCAGCTGTTCA-3′ |
DF1-ATF4-R | 5’-ACGGTGGCTTCCAGATGTTCCATA-3’ [60] | DF1-GRP78-R | 5’-ATGCCAAGTGTCAGAGGACACACA-3’ [60] |
DF1-ACTINF | 5’-CTGTGCCCATCTATGAAGGCTA-3′ |  |  |
DF1-ACTINR | 5’-ATTTCTCTCTCGGCTGTGGTG-3′ |  |  |