Table 1 Summary of antibodies and conditions of use
Antigen | Clone and origin | Dilution, incubation time | Source, reference | Antigen retrieval |
|---|---|---|---|---|
ERα | C311 mouse monoclonal | 1:50 44 minutes | Santa Cruz Biotechnology, sc-787 | None |
PR | 10A9 mouse monoclonal | 1:50 1 h40 | Meridian Life Science, K42546 M | HIER, CC1, 1 hour |
HER2 | 4B5 rabbit monoclonal | Prediluted 8 minutes | Roche Diagnostics, 790–2991 | HIER, CC1, 30 minutes |
Ki-67 | MIB1 mouse monoclonal | 1:50 32 minutes | Dako, M7240 | HIER, CC1, 1 hour |
Cytokeratins 5/6 | D5/16 B4 mouse monoclonal | 1:50 16 minutes | Dako, M7237 | HIER, CC1, 30 minutes |
Bcl-2 | 7/Bcl-2 mouse monoclonal | 1:50 44 minutes | BD Transduction laboratories, 610,539 | HIER, CC1, 24 minutes |