Table 1 Summary of antibodies and conditions of use
| Antigen | Clone and origin | Dilution, incubation time | Source, reference | Antigen retrieval |
|---|---|---|---|---|
| ERα |
C311 mouse monoclonal |
1:50 44 minutes | Santa Cruz Biotechnology, sc-787 | None |
| PR |
10A9 mouse monoclonal |
1:50 1 h40 | Meridian Life Science, K42546 M | HIER, CC1, 1 hour |
| HER2 |
4B5 rabbit monoclonal |
Prediluted 8 minutes | Roche Diagnostics, 790–2991 | HIER, CC1, 30 minutes |
| Ki-67 |
MIB1 mouse monoclonal |
1:50 32 minutes | Dako, M7240 | HIER, CC1, 1 hour |
| Cytokeratins 5/6 |
D5/16 B4 mouse monoclonal |
1:50 16 minutes | Dako, M7237 | HIER, CC1, 30 minutes |
| Bcl-2 |
7/Bcl-2 mouse monoclonal |
1:50 44 minutes | BD Transduction laboratories, 610,539 | HIER, CC1, 24 minutes |
