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Table 1 Summary of antibodies and conditions of use

From: Bcl-2 expression and prognostic significance in feline invasive mammary carcinomas: a retrospective observational study

Antigen Clone and origin Dilution, incubation time Source, reference Antigen retrieval
ERα C311
mouse monoclonal
1:50
44 minutes
Santa Cruz Biotechnology, sc-787 None
PR 10A9
mouse monoclonal
1:50
1 h40
Meridian Life Science, K42546 M HIER, CC1, 1 hour
HER2 4B5
rabbit monoclonal
Prediluted
8 minutes
Roche Diagnostics, 790–2991 HIER, CC1, 30 minutes
Ki-67 MIB1
mouse monoclonal
1:50
32 minutes
Dako, M7240 HIER, CC1, 1 hour
Cytokeratins 5/6 D5/16 B4
mouse monoclonal
1:50
16 minutes
Dako, M7237 HIER, CC1, 30 minutes
Bcl-2 7/Bcl-2
mouse monoclonal
1:50
44 minutes
BD Transduction laboratories, 610,539 HIER, CC1, 24 minutes
  1. CC1 cell conditioning solution 1, Ventana Medical Systems (reference 950–124)
  2. HIER heat-induced epitope retrieval