A gD&gC-substituted pseudorabies virus vaccine strain provides complete clinical protection and is helpful to prevent virus shedding against challenge by a Chinese pseudorabies variant

Table 2 Primers for PCR or sequencing

Primer	Sequence (5′- 3′)
Bartha-gC-H1-F	CATGAATTCGCGTCGACGATGCTGCTCGG
Bartha-gC-H1-R	TCAGGTACCGTTAATTAACAAAAACGACGCGAGCGTGGG
Bartha-gC-H2-F	CGAGGATCCGTTAATTAACCACGTCGAATCAATAAACG
Bartha-gC-H2-R	CACAAGCTTCCGTGAACAACATGCTGCTG
AH02LA-gD-F	TCGATCTACATCTGCGTCGC
AH02LA-gD-R	ATCATCGACGCCGGTACTGC
Kan ins gD F	TCCGTCGACGGCGTGAACATCCTCACCGACTTCATGGTGGCGCTCCCCGGGATGACGACGATAAGTAGGGATAAC
Kan ins gD R	TACGTCGACGGGTAATGCCAGTGTTACAACCA
Bartha ins gD^A-kan F	CATGCTGCTCGCAGCGCTAT
Bartha ins gD^A-kan R	GCCGGTACTGCGGAGGCTAC
AH02LA gC flanking F	GCGTCGACGATGCTGCTCGG
AH02LA gC flanking R	CCGTGAACAACATGCTGCTG
SEQ-AH02LA gC F	ACTTTTTCACGACGCCGCGG
SEQ-AH02LA gC R	AAGCGGACCTCGAAGGTCTC

ISSN: 1746-6148