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Fig. 1 | BMC Veterinary Research

Fig. 1

From: A gD&gC-substituted pseudorabies virus vaccine strain provides complete clinical protection and is helpful to prevent virus shedding against challenge by a Chinese pseudorabies variant

Fig. 1

Construction of mini-F recombinant PRV Bartha strain (PRV B-mini-F), gD substituted clone (pB-gDS-mini-F) and gC&gD-substituted virus (PRV B-gD&gCS) (a) Mini-F was inserted in lieu of gC to generate the mini-F recombinant PRV Bartha-K61 strain for BAC through homologous recombination. b AH02LA gD-KAN was inserted in lieu of gD in the Bartha genome through the first recombination, the kanamycin gene was deleted in the second step, generating gD substituted clone (pB-gDS-mini-F). c Another recombination was performed to substitute the mini-F sequence with gC of AH02LA, generating gC&gD substituted virus (PRV B-gD&gCS)

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