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Table 1 Primers used in this study

From: Characterization of the responses of the caspase 2, 3, 6 and 8 genes to immune challenges and extracellular ATP stimulation in the Japanese flounder (Paralichthys olivaceus)

Primer name

Sequences (5’→ 3’)

Applications

JfCaspase 2-f

GGAGCAGCTGGACGATCGAC

Gene cloning

JfCaspase 2-r

ATATGTGCATGTGATCGATA

 

JfCaspase 3-f

CAACAACAAGAACTTCGACAGG

JfCaspase 3-r

TGTATATGTCAGGACAGTGCAA

JfCaspase 6-f

CATTCGGAAGGAGAGAGGGA

JfCaspase 6-r

GTGCTCAGCAACGACATACAG

JfCaspase 8-f

CTGGCTTGTGTGGGAGGGAG

JfCaspase 8-r

CAGCAATCTGTATCATACAGG

qJfCaspase 2-f

CCTCGTGGTTTCGCCTTG

qRT-PCR

qJfCaspase 2-r

CGGTGGTCTGGTCGTTGG

 

qJfCaspase 3-f

TCGCTGCAAATCGCTGGT

qJfCaspase 3-r

CTGTGGAGAAGGCGTAGAGGA

qJfCaspase 6-f

CTGAGCCACGGTGAGAACG

qJfCaspase 6-r

ATTGTCCACGGCATCGCA

qJfCaspase 8-f

CAGAGCCCTTCACGAGCAA

qJfCaspase 8-r

CAAGGCACCGTCTCACCAT

beta-actin-f

AGGTTCCGTTGTCCCG

 

beta-actin-r

TGGTTCCTCCAGATAGCAC

  1. f and r denote forward and reverse primer, respectively
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BMC Veterinary Research

ISSN: 1746-6148

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