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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Characterization of the responses of the caspase 2, 3, 6 and 8 genes to immune challenges and extracellular ATP stimulation in the Japanese flounder (Paralichthys olivaceus)

Fig. 4

LPS- and poly(I:C)-induced JfCaspase 1, 2, 3, 6 and 8 gene expression in Japanese flounder HKMs. Overnight-cultured Japanese flounder HKMs were stimulated with 20 μg/ml LPS (left column) or poly(I:C) (right column) for the indicated times. Total RNA from the cells was purified and transcribed into cDNA. JfCaspase 1, 2, 3, 6 and 8 gene expression changes compared with the expression in the untreated controls were determined by qRT-PCR analysis. The values are presented as the means ± standard deviation (n = 3)

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BMC Veterinary Research

ISSN: 1746-6148

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