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Table 1 Sensitivities of the 3 cell cultures to ORFV

From: Comparison of the sensitivity of three cell cultures to ORFV

Cell cultures

CPE occurrence time (h)

Time to 75% CPE (h)

Virus titers at 75% CPE (log TCID50/0.1 ml)

MDBK cells

36.16 ± 0.29

96.00 ± 1.00

5.10 ± 0.10

Primary neonatal Bovine testicular cells

24.33 ± 0.29a

72.5 ± 0.5

5.50 ± 0.10

Bovine Sertoli cells

24.50 ± 0.53*

65.50 ± 0.50*

6.55 ± 0.58*

  1. The bovine Sertoli cells from the 3rd passage were used in this experiment. Data are expressed as mean ± S.D. (n = 3, each group). Statistically significant differences between bovine Sertoli cells and MDBK cells and bovine Sertoli cells and primary neonatal Bovine testicular cells are indicated by *(P ≤ 0.05). The Mann-Whitney U test was used to compare the results of each group
  2. aNo differences were found between bovine Sertoli cells and primary neonatal bovine testicular cells