Cell cultures
|
CPE occurrence time (h)
|
Time to 75% CPE (h)
|
Virus titers at 75% CPE (log TCID50/0.1 ml)
|
---|
MDBK cells
|
36.16 ± 0.29
|
96.00 ± 1.00
|
5.10 ± 0.10
|
Primary neonatal Bovine testicular cells
|
24.33 ± 0.29a
|
72.5 ± 0.5
|
5.50 ± 0.10
|
Bovine Sertoli cells
|
24.50 ± 0.53*
|
65.50 ± 0.50*
|
6.55 ± 0.58*
|
- The bovine Sertoli cells from the 3rd passage were used in this experiment. Data are expressed as mean ± S.D. (n = 3, each group). Statistically significant differences between bovine Sertoli cells and MDBK cells and bovine Sertoli cells and primary neonatal Bovine testicular cells are indicated by *(P ≤ 0.05). The Mann-Whitney U test was used to compare the results of each group
- aNo differences were found between bovine Sertoli cells and primary neonatal bovine testicular cells