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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Comparison of the sensitivity of three cell cultures to ORFV

Fig. 2

Identification results of the 3rd passage of the bovine Sertoli cells after isolation and purification by HE staining, and indirect immunofluorescence staining. HE staining assay (nucleus was indicated by 1 arrow) (a); FasL protein (indicated by 1 arrow) detection in the bovine Sertoli cells (b) and primary neonatal bovine testicular cells by immunohistochemical assay (c). Negative control (d). Bovine Sertoli cells and primary neonatal bovine testicular cells cultured for 96 h were visualized with bright-field and fluorescent microscopy, and the resulting images were merged. Ninety-five percent of the bovine Sertoli cells and 78% of the primary neonatal bovine testicular cells exhibited fluorescence, respectively

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