Fig. 1From: Inherited selective cobalamin malabsorption in Komondor dogs associated with a CUBN splice site variantUrine protein analysis of Komondor littermates. Urine samples containing 200 μg creatinine were desalted and concentrated by centrifugation through a 10 kDa molecular weight cutoff membrane. Proteins were separated by 15% SDS-PAGE and visualized by silver staining. Lane 1 shows urine proteins obtained from an affected dog (case 5, Additional file 1) after cobalamin replenishment and during metabolic remission. Lane 2 shows urine proteins from a heterozygous but clinically healthy littermate of case 5Back to article page