Fig. 5From: Cytoplasmic glycoengineering of Apx toxin fragments in the development of Actinobacillus pleuropneumoniae glycoconjugate vaccinesApx toxin fragments expressed in E. coli with and without chromosomally encoded ngt and agt, were resolved by SDS-PAGE and detected by immunoblot analysis probed with anti-6xHis (green) and anti-dextran (red) antibody. Lanes 2/3: ApxIAD3 (G71 T); lanes 4/5: ApxIAD3 (V83 T); lanes 6/7: ApxIAD3 (G114 T); lanes 8/9: ApxIIAD3 (G64 T); lanes 10/11: ApxIAD1 (whole cell lysate); lane 12: E. coli expressing AtaC1866–2428 and plasmid encoded ngt and agtBack to article page