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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Cytoplasmic glycoengineering of Apx toxin fragments in the development of Actinobacillus pleuropneumoniae glycoconjugate vaccines

Fig. 5

Apx toxin fragments expressed in E. coli with and without chromosomally encoded ngt and agt, were resolved by SDS-PAGE and detected by immunoblot analysis probed with anti-6xHis (green) and anti-dextran (red) antibody. Lanes 2/3: ApxIAD3 (G71 T); lanes 4/5: ApxIAD3 (V83 T); lanes 6/7: ApxIAD3 (G114 T); lanes 8/9: ApxIIAD3 (G64 T); lanes 10/11: ApxIAD1 (whole cell lysate); lane 12: E. coli expressing AtaC1866–2428 and plasmid encoded ngt and agt

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