Detection of endoplasmic reticulum stress and the unfolded protein response in naturally-occurring endocrinopathic equine laminitis

Cassimeris, Lynne; Engiles, Julie B.; Galantino-Homer, Hannah

doi:10.1186/s12917-018-1748-x

Table 1 Sample data summary of signalment, laminitis risk factor, pathology category, and ER stress/UPR marker data

From: Detection of endoplasmic reticulum stress and the unfolded protein response in naturally-occurring endocrinopathic equine laminitis

Samples (case #, limb)	Age (yr)	Breed	Sex	Laminitis Risk Factor	Laminitis Category	Immunoblot Relative Band Intensities (arbitrary units)			Grp78/BiP Localization
Samples (case #, limb)	Age (yr)	Breed	Sex	Laminitis Risk Factor	Laminitis Category	XBP1s	Grp78/BiP	Grp94	Grp78/BiP Localization
Control
61 RF	11	WB	MC	NL/O	SUB	26.8	15.7	12	nd
92 LF	18	WB	F	NL/O	SUB	24.7	11.4	17.1	nd
102 LF	17	QH	F	NL/O	NL	38.4	0.9	25	negative
110 LF	14	TB	MC	NL/O	NL	9.7	nd	17.2	nd
111 LF	18	TB	F	NL/O	NL	nd	nd	nd	negative
113 LF	12	TB	F	NL/O	NL	27.6	0.5	17.2	negative
114 LF	12	TB	F	NL/O	NL	5.2	2.9	26.5	negative
129 RF	22	AR	F	NL/IF	NL	5.8	18.4	42.8	negative
Mean ± sd						19.7 ± 12.9	8.3 ± 7.9	20.8 ± 10.7
EL Front Limb
63 RF	33	MO	F	PPID	SEV	79.1	12.8	17.4	nd
63 LF	33	MO	F	PPID	SEV	nd	nd	nd	AbAx
73 LF	6	TBX	F	OB/RA	MOD	36.6	20.5	13.2	negative
75 RF	22	MO	F	PPID	SEV	96.5	250	106	nd
75 LF	22	MO	F	PPID	SEV	nd	nd	nd	AbAx, Mid (KA)
90 LF	21	MH	M	OB/RA	MOD	54.3	1.9	41.5	nd
101 RF	13	MO	MC	OB/RA	SEV	16	79	91.5	AbAx, Mid (KA)
104 RF	23	WB	MC	PPID	SEV	36.6	1.3	24.5	AbAx, Mid (KA)
109 LF	9	QH	F	OB/RA	SEV	19	23.6	27.9	AbAx
116 LF	8	QH	F	OB/RA	SEV	100	131.2	100	nd
116 RF	8	QH	F	OB/RA	SEV	nd	nd	nd	AbAx, Mid (KA)
134 RF	18	TB	MC	PPID	SEV	87.3	100	57.9	negative
134 LF	18	TB	MC	PPID	SEV	nd	nd	nd	AbAx, Mid (KA)
140 LF	14	TBX	F	OB/RA	SEV	12	15.1	36.9	AbAx, Mid (KA)
141 LF	21	RMH	F	PPID	SEV	72.6	296	70.1	AbAx, Mid (KA)
141 RF	21	RMH	F	PPID	SEV	nd	nd	nd	AbAx, Mid (KA)
165 LF	23	WB	F	PPID	MOD	23	15	31.6	nd
Mean ± sd						52.8 ± 33.0	78.9 ± 100.2	51.5 ± 32.9
EL Hind Limb
63 LH	33	MO	F	PPID	SUB	nd	nd	nd	negative
73 LH	6	TBX	F	OB/RA	NL	19.4	15	39.7	negative
75 RH	22	MO	F	PPID	NL	17.8	1.7	33	negative
101 LH	13	MO	MC	OB/RA	SUB	nd	nd	nd	negative
104 RH	23	WB	MC	PPID	SUB	7.7	7.6	21.1	nd
109 RH	9	QH	F	OB/RA	NL	nd	4.7	27	negative
116 RH	8	QH	F	OB/RA	SUB	1.8	5.2	17.4	negative
134 RH	18	TB	MC	PPID	NL	3.8	33	25.4	negative
141 RH	21	RMH	F	PPID	NL	0.1	5.5	17.7	nd
165 LH	23	WB	F	PPID	NL	9.4	5.4	23.8	nd
Mean ± sd						8.6 ± 7.6	9.8 ± 10.2	25.7 ± 7.6

legend: Summary of EL cases and controls used in the present study. Case numbers were assigned at the time of tissue collection, with foot identified as left front (LF), right front (RF), left hind (LH), or right hind (RH). Age listed in years. Breeds include: American Quarter Horse (QH); Arabian (AR); Miniature Horse (MH); Morgan (MO); Rocky Mountain Horse (RMH); Thoroughbred (TB); Thoroughbred cross (TBX; case 73 is TB/Clydesdale cross; case 140 is TB/WB cross); Warmblood (WB). Sex is listed as female (F), male (M), or male castrate (MC). Diagnosis includes non-laminitic, other orthopedic issues (NL/O); non-laminitic, infertility (NL/IF); PPID; obesity/regional adiposity (OB/RA). Laminitis categories include non-laminitic (NL); sub-clinical (SUB), Moderate chronic-active (MOD), and Severe chronic-active (SEV) based on the criteria presented in Table 2 and Methods. Relative immunoblot band intensities were normalized and calculated as described in methods. The mean ± standard deviation (sd) is given for each marker. EL Front Limbs differed from Controls (p = 0.007 (XBP1s), 0.03 (Grp78/BiP), 0.009 Grp94) and from EL Hind Limbs (p = 0.006 (XBP1s), 0.05 (Grp78/BiP). Anti-Grp78/BiP labeled sections were scored visually for detectable expression. Positive antibody labeling is noted by location along the PEL axis: abaxial (AbAx), middle (Mid), keratinized axis (KA). nd, not determined

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