Skip to main content

Table 1 Sample data summary of signalment, laminitis risk factor, pathology category, and ER stress/UPR marker data

From: Detection of endoplasmic reticulum stress and the unfolded protein response in naturally-occurring endocrinopathic equine laminitis

Samples (case #, limb) Age (yr) Breed Sex Laminitis Risk Factor Laminitis Category Immunoblot Relative Band Intensities (arbitrary units) Grp78/BiP Localization
XBP1s Grp78/BiP Grp94
Control
61 RF 11 WB MC NL/O SUB 26.8 15.7 12 nd
92 LF 18 WB F NL/O SUB 24.7 11.4 17.1 nd
102 LF 17 QH F NL/O NL 38.4 0.9 25 negative
110 LF 14 TB MC NL/O NL 9.7 nd 17.2 nd
111 LF 18 TB F NL/O NL nd nd nd negative
113 LF 12 TB F NL/O NL 27.6 0.5 17.2 negative
114 LF 12 TB F NL/O NL 5.2 2.9 26.5 negative
129 RF 22 AR F NL/IF NL 5.8 18.4 42.8 negative
Mean ± sd       19.7 ± 12.9 8.3 ± 7.9 20.8 ± 10.7  
EL Front Limb
63 RF 33 MO F PPID SEV 79.1 12.8 17.4 nd
63 LF 33 MO F PPID SEV nd nd nd AbAx
73 LF 6 TBX F OB/RA MOD 36.6 20.5 13.2 negative
75 RF 22 MO F PPID SEV 96.5 250 106 nd
75 LF 22 MO F PPID SEV nd nd nd AbAx, Mid (KA)
90 LF 21 MH M OB/RA MOD 54.3 1.9 41.5 nd
101 RF 13 MO MC OB/RA SEV 16 79 91.5 AbAx, Mid (KA)
104 RF 23 WB MC PPID SEV 36.6 1.3 24.5 AbAx, Mid (KA)
109 LF 9 QH F OB/RA SEV 19 23.6 27.9 AbAx
116 LF 8 QH F OB/RA SEV 100 131.2 100 nd
116 RF 8 QH F OB/RA SEV nd nd nd AbAx, Mid (KA)
134 RF 18 TB MC PPID SEV 87.3 100 57.9 negative
134 LF 18 TB MC PPID SEV nd nd nd AbAx, Mid (KA)
140 LF 14 TBX F OB/RA SEV 12 15.1 36.9 AbAx, Mid (KA)
141 LF 21 RMH F PPID SEV 72.6 296 70.1 AbAx, Mid (KA)
141 RF 21 RMH F PPID SEV nd nd nd AbAx, Mid (KA)
165 LF 23 WB F PPID MOD 23 15 31.6 nd
Mean ± sd       52.8 ± 33.0 78.9 ± 100.2 51.5 ± 32.9  
EL Hind Limb
63 LH 33 MO F PPID SUB nd nd nd negative
73 LH 6 TBX F OB/RA NL 19.4 15 39.7 negative
75 RH 22 MO F PPID NL 17.8 1.7 33 negative
101 LH 13 MO MC OB/RA SUB nd nd nd negative
104 RH 23 WB MC PPID SUB 7.7 7.6 21.1 nd
109 RH 9 QH F OB/RA NL nd 4.7 27 negative
116 RH 8 QH F OB/RA SUB 1.8 5.2 17.4 negative
134 RH 18 TB MC PPID NL 3.8 33 25.4 negative
141 RH 21 RMH F PPID NL 0.1 5.5 17.7 nd
165 LH 23 WB F PPID NL 9.4 5.4 23.8 nd
Mean ± sd       8.6 ± 7.6 9.8 ± 10.2 25.7 ± 7.6  
  1. legend: Summary of EL cases and controls used in the present study. Case numbers were assigned at the time of tissue collection, with foot identified as left front (LF), right front (RF), left hind (LH), or right hind (RH). Age listed in years. Breeds include: American Quarter Horse (QH); Arabian (AR); Miniature Horse (MH); Morgan (MO); Rocky Mountain Horse (RMH); Thoroughbred (TB); Thoroughbred cross (TBX; case 73 is TB/Clydesdale cross; case 140 is TB/WB cross); Warmblood (WB). Sex is listed as female (F), male (M), or male castrate (MC). Diagnosis includes non-laminitic, other orthopedic issues (NL/O); non-laminitic, infertility (NL/IF); PPID; obesity/regional adiposity (OB/RA). Laminitis categories include non-laminitic (NL); sub-clinical (SUB), Moderate chronic-active (MOD), and Severe chronic-active (SEV) based on the criteria presented in Table 2 and Methods. Relative immunoblot band intensities were normalized and calculated as described in methods. The mean ± standard deviation (sd) is given for each marker. EL Front Limbs differed from Controls (p = 0.007 (XBP1s), 0.03 (Grp78/BiP), 0.009 Grp94) and from EL Hind Limbs (p = 0.006 (XBP1s), 0.05 (Grp78/BiP). Anti-Grp78/BiP labeled sections were scored visually for detectable expression. Positive antibody labeling is noted by location along the PEL axis: abaxial (AbAx), middle (Mid), keratinized axis (KA). nd, not determined