Fig. 5

Positions of Grp78/BiP positive staining cells in lamellar tissue. All images are from laminitic samples. Grp78/BiP (green) and WGA (red) were localized as described in Methods. Images were selected to show the range of Grp78/BiP staining patterns observed in laminitic tissues. In the Abaxial region, Grp78/BiP expressing cells (yellow arrows) are found in multiple layers of suprabasal cells, or limited to a single layer of cells adjacent to the keratinized axis. In the middle region, Grp78/BiP positive cells (yellow arrows) are found adjacent to the keratinized axis (KA), often several cell layers deep. In some cases, positive cells are found along the length of the keratinized axis, while in other tissues expression is limited to groups of cells. All images are shown at the same magnification. Scale bar is 50 μm