Fig. 3

Grp94 is expressed at higher levels in many laminitic samples. a. Representative immunoblots of lamellar tissue from control (C), EL front limbs (F) and EL hind (H) limbs. The anti-Grp94 antibody recognizes a band of the same mw in Hela (human) cells, and this band is upregulated by treatment with tunicamycin, an inducer of ER stress (see Methods). b. Box plot of normalized Grp94 immunoblot band intensities showing higher expression in laminitic EL front limbs. Horizontal lines represent the median value for each group. c Comparison of Grp94 expression in paired EL front and hind limbs from the same horses (n = 8). The inset shows an expanded region of the Y axis