From: Sudden death due to gas gangrene caused by Clostridium septicum in goats
Target gene | Sequences (5′-3′) (amplicon sizes) | PCR conditions | References |
---|---|---|---|
C. septicum flagellin gen (fliC) | FlaF- AGAATAAACAGAAGCTGGAGATG FlaseR-TTTATTGAATTGTGTTTGTGAAG (Amplicon:294 bp) | 94 °C 1 min 55 °C 1 min 72 °C 90 s (30 cycles) | [12] |
C. chauvoei flagellin gen (fliC) | FlaF- AGAATAAACAGAAGCTGGAGATG FlachR-TACTAGCAGCATCAAATGTACC (Amplicon:535 bp) | ||
Alpha toxin Gen | F-AATTCAGTGTGCGGCAGTAG R-CCTGCCCCAACTTCTCTTTT (Amplicon:270 bp) | 94 °C 1 min 55 °C 1 min 72 °C 1 min (35 cycles) | [13] |