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Table 1 Primers and PCR conditions used in this study

From: Sudden death due to gas gangrene caused by Clostridium septicum in goats

Target gene

Sequences (5′-3′) (amplicon sizes)

PCR conditions

References

C. septicum flagellin gen (fliC)

FlaF- AGAATAAACAGAAGCTGGAGATG

FlaseR-TTTATTGAATTGTGTTTGTGAAG

(Amplicon:294 bp)

94 °C 1 min

55 °C 1 min

72 °C 90 s

(30 cycles)

[12]

C. chauvoei flagellin gen (fliC)

FlaF- AGAATAAACAGAAGCTGGAGATG

FlachR-TACTAGCAGCATCAAATGTACC

(Amplicon:535 bp)

Alpha toxin Gen

F-AATTCAGTGTGCGGCAGTAG

R-CCTGCCCCAACTTCTCTTTT

(Amplicon:270 bp)

94 °C 1 min

55 °C 1 min

72 °C 1 min

(35 cycles)

[13]