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Table 1 Development to the blastocyst stage and total blastomere number

From: N-acetylcysteine addition after vitrification improves oocyte mitochondrial polarization status and the quality of embryos derived from vitrified murine oocytes

Oocyte treatment n Blastocyst rate (%) n Cell count
Fresh control 67 90.7 ± 1.8a 12 86.8 ± 2.5a
Vitrified Control 92 85.9 ± 1.5a,b 15 58.9 ± 2.5b
Vitrified NAC-Pre 81 79.1 ± 1.8b 12 36.7 ± 2.3c
Vitrified NAC-Post 80 90.1 ± 1.8a,c 12 76.8 ± 4.1d
  1. Development to the blastocyst stage and total cell number of embryos obtained by IVF from fresh or vitrified murine oocytes in presence or absence of NAC. The groups studied were: F-C: oocytes were cultured in KSOM medium for 2 h prior to IVF; V-C: oocytes were cultured in KSOM medium for 2 h prior to vitrification. After warming, oocytes were allowed to recover in KSOM medium for 2 h and subjected to IVF afterwards; V-NAC-Pre: oocytes were cultured in KSOM medium supplemented with 1 mM NAC for 2 h prior to vitrification. Warmed oocytes were allowed to recover for 2 h in KSOM medium before IVF; V-NAC-Post: oocytes were cultured in KSOM medium for 2 h prior to vitrification. Warmed oocytes were allowed to recover for 2 h in KSOM medium added with 1 mM NAC before IVF. Values bearing different letters in the same column differ statistically (p < 0.05); values are expressed as the mean ± SEM.