|
Oocyte treatment
|
n
|
Blastocyst rate (%)
|
n
|
Cell count
|
|---|
|
Fresh control
|
67
|
90.7 ± 1.8a
|
12
|
86.8 ± 2.5a
|
|
Vitrified Control
|
92
|
85.9 ± 1.5a,b
|
15
|
58.9 ± 2.5b
|
|
Vitrified NAC-Pre
|
81
|
79.1 ± 1.8b
|
12
|
36.7 ± 2.3c
|
|
Vitrified NAC-Post
|
80
|
90.1 ± 1.8a,c
|
12
|
76.8 ± 4.1d
|
- Development to the blastocyst stage and total cell number of embryos obtained by IVF from fresh or vitrified murine oocytes in presence or absence of NAC. The groups studied were: F-C: oocytes were cultured in KSOM medium for 2 h prior to IVF; V-C: oocytes were cultured in KSOM medium for 2 h prior to vitrification. After warming, oocytes were allowed to recover in KSOM medium for 2 h and subjected to IVF afterwards; V-NAC-Pre: oocytes were cultured in KSOM medium supplemented with 1 mM NAC for 2 h prior to vitrification. Warmed oocytes were allowed to recover for 2 h in KSOM medium before IVF; V-NAC-Post: oocytes were cultured in KSOM medium for 2 h prior to vitrification. Warmed oocytes were allowed to recover for 2 h in KSOM medium added with 1 mM NAC before IVF. Values bearing different letters in the same column differ statistically (p < 0.05); values are expressed as the mean ± SEM.
