Fig. 4

Representative micrographs of expanded blastocysts stained with Hoechst 33342 derived from fresh and vitrified murine oocytes after IVF. The letters in each panel represent: a) Fresh oocytes cultured in KSOM medium for 2 h; b) Vitrified oocytes cultured in KSOM medium for 2 h after warming; c) oocytes cultured in KSOM medium supplemented with 1 mM of NAC for 2 h prior to vitrification and cultured in KSOM medium for 2 h after warming; d) Vitrified oocytes cultured in KSOM medium supplemented with 1 mM of NAC for 2 h after warming. The scale represents 100 μm; the micrographs were taken using a 40 × objective