Method | Sitting-drop vapor diffusion |
---|---|
Temperature (°C) | 4 |
Protein concentration (mg/mL) | 15 |
Buffer composition of protein solution | 50 mM NaCl, 20 mM Tris–HCl pH 8.0 |
Composition of reservoir solution | 0.1 M BIS-TRIS pH 6.5, 0.2 M Ammonium acetate, 25% (w/v) PEG 3350 |
Volume and ratio of drop | 1 μL protein solution mixed with 1 μL reservoir solution |
Volume of reservoir (μL) | 120 |