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Table 2 Crystallizing condition

From: Expression, purification, crystallization and preliminary X-ray diffraction analysis of swine leukocyte antigen 2 complexed with a CTL epitope AS64 derived from Asia1 serotype of foot-and-mouth disease virus

Method Sitting-drop vapor diffusion
Temperature (°C) 4
Protein concentration (mg/mL) 15
Buffer composition of protein solution 50 mM NaCl, 20 mM Tris–HCl pH 8.0
Composition of reservoir solution 0.1 M BIS-TRIS pH 6.5, 0.2 M Ammonium acetate, 25% (w/v) PEG 3350
Volume and ratio of drop 1 μL protein solution mixed with 1 μL reservoir solution
Volume of reservoir (μL) 120