Skip to main content
Fig. 1 | BMC Veterinary Research

Fig. 1

From: Expression, purification, crystallization and preliminary X-ray diffraction analysis of swine leukocyte antigen 2 complexed with a CTL epitope AS64 derived from Asia1 serotype of foot-and-mouth disease virus

Fig. 1

Purification of the refolded SLA-2*HB01-AS64-sβ2m by FPLC Superdex 200 16/60 Hi-Load gel-filtration and anion-exchange chromatography Resource Q (GE Healthcare). a Gel-filtration profile of the refolded SLA-2*HB01-AS64-sβ2m products. Peak 1 represents aggregated heavy chains of SLA-2*HB01, peaks 2–6 represent correctly refolded SLA-2*HB01-AS64-sβ2m complex (~ 44 kDa) and peak 7 represents surplus sβ2m. Inset: SDS–PAGE gel detection (15%) for peaks 1, 2–6 and 7. M, the low molecular-weight protein ladder (kDa) with 97.1, 66.3, 43.0, 31.0, 20.1 and 14.4. b Further purification of the refolded SLA-2*HB01-AS64-sβ2m by anion-exchange chromatography Resource Q. Peak 1 contains aggregated light chain of sβ2m, peaks 2–4 represent the refolded SLA-2*HB01-AS64-sβ2m products, which was eluted with a NaCl concentration of 21.5–26.5%. Inset: SDS-PAGE gel detection (15%) for peaks 1 and 2–4. M, the low molecular-weight protein ladder (kDa) with 97.1, 66.3, 43.0, 31.0, 20.1 and 14.4

Back to article page

BMC Veterinary Research

ISSN: 1746-6148

Contact us