Fig. 1

PCA score plots (a, c, e, and g) and OPLS-DA score plots (b, d, f and h) of rumen and plasma samples obtained from beef steers fed the 3 different diets. On the score plot, each point represents an individual sample. a and b are derived from the POS of the rumen fluid, and c and d are derived from the NEG of the rumen fluid. e and f are derived from the POS of plasma, and g and h are derived from the NEG of plasma. LA (circle) is the treatment diet based on corn grain steeped for 48 h in an equal quantity of tap water containing 1% lactic acid (wt/vol), HA (square) is the treatment diet based on corn grain steeped for 48 h in an equal quantity of tap water containing 1% hydrochloric acid (wt/vol), and CON (triangle) is the control diet containing corn grain steeped for 48 h in an equal quantity of tap water