Development of a novel oil-in-water emulsion and evaluation of its potential adjuvant function in a swine influenza vaccine in mice

Table 3 Lung virus isolation from mice of different groups challenge with the homologous virus

Group	Number of mice carrying H3N2 virus in lung post challenge/total number (Positive rate)
EAS + antigen	0/5
MF59 + antigen	0/5
W/O + antigen	0/5
antigen	1/5
mock	4/5

Mice were challenged intranasally with 0.2 × 10⁵ EID50 of the H3N2 subtype virus strain A/swine/Shandong/3/2005 two weeks after the second immunization. Five mice of each group were euthanized at 5 days post infection (dpi), and whole lungs were collected and homogenized in PBS. The homogenates were centrifuged and the supernatant from each sample was collected for passage in 10-day-old specific pathogen free (SPF) embryonated chicken eggs. The allantoic fluid was harvested and tested for hemagglutinin activity

ISSN: 1746-6148