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Table 2 Heat Stability Study of EAS at 4 °C, 25 °C and 37 °C (values are shown as means ±SD, n = 3)

From: Development of a novel oil-in-water emulsion and evaluation of its potential adjuvant function in a swine influenza vaccine in mice

EAS Time (months)
T0 1 3 6 9 12 18 24
4 °C Particle size 104 ± 2 104 ± 2 101 ± 3 105 ± 1 104 ± 2 105 ± 2 107 ± 2 106 ± 2
Zeta potential −38.8 ± 0.1 −38.3 ± 0.4 − 38.5 ± 0.2 − 38.8 ± 0.2 − 38.7 ± 0.2 −38.5 ± 0.2 − 38.7 ± 0.02 −38.3 ± 0.3
pH 6.8 ± 0.1 6.7 ± 0.2 6.8 ± 0.1 6.6 ± 0.3 6.8 ± 0.1 6.8 ± 0.1 6.7 ± 0.3 6.7 ± 0.2
Viscosity 2.01 ± 0.14 2.01 ± 0.21 1.90 ± 0.17 1.94 ± 0.17 2.04 ± 0.15 2.11 ± 0.01 1.99 ± 0.04 2.03 ± 0.12
25 °C Particle size 104 ± 2 103 ± 3 109 ± 1 114 ± 2 141 ± 9 ND ND ND
Zeta potential −38.3 ± 0.1 −38.4 ± 0.5 −38.7 ± 0.3 −38.5 ± 0.5 −35.4 ± 0.4 ND ND ND
pH 6.8 ± 0.2 6.5 ± 0.3 6.8 ± 0.1 6.5 ± 0.3 6.6 ± 0.2 ND ND ND
Viscosity 2.01 ± 0.05 2.04 ± 0.01 2.24 ± 0.23 2.17 ± 0.05 2.43 ± 0.22 ND ND ND
37 °C Particle size 105 ± 2 107 ± 2 115 ± 2 157 ± 12 ND ND ND ND
Zeta potential −38.6 ± 0.3 −38.4 ± 0.2 −36.9 ± 0.3 −35.8 ± 0.4 ND ND ND ND
pH 6.8 ± 0.2 6.5 ± 0.3 6.6 ± 0.2 6.7 ± 0.2 ND ND ND ND
Viscosity 2.03 ± 0.11 2.11 ± 0.18 2.34 ± 0.19 2.55 ± 0.38 ND ND ND ND